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Nanodrop 2000 (Thermo Scientific)

Cost: 0.10$/sample

A spectrophotometer (full-spectrum and UV-Vis spectrophotometers) used to quantify and assess purity of DNA, Ï㽶ÊÓƵ, protein and more!

We offer training, calibration and maintenance of the spectrophotometer.

Guidelines

Before using it, you should have a training (10-15 minutes) with a member of the C- BIG Repository team.

The training will cover:

• the use of the nanodrop
• the cleanup before and after use
• the software

After training, a password will be given to you to access the nanodrop.

Enspire Multimode Plate Reader (Perkin Elmer)

Cost: Free for now

This plate reader is a high performance, compact and configurable instrument designed with filter/quadmonochromator capabilities. It has an intuitive and ‘easy-to-use’ touch screen to reduce assay start-up times and simplify protocols.

For now, the Enspire plate reader is free to use, but in a near future a price will be determined which will allow us to give you more services, i.e. training and maintenance.

Guidelines

For now, please sign the log in sheet available on the reader. Extra sheets are available next to it.

Before using it, you should have a training with a member of the C- BIG Repository team (the complete training will be available soon).

The training will cover:

• the use of the Enspire
• the cleanup before and after use
• the software

Fluorescence Zeiss Microscope​

DetailsÌýavailableÌýsoon​

Elipsot

Details available soon

MetaExpress

Details available soon

Z Counter

Details available soon

SOPs

If any inquiries please contact us directly at cbig.mni [at] mcgill.ca

Ìý

³Ò±ð²Ô±ð°ù²¹±ô:​

• C-BIG-01-001 Administration of Standard Operating Procedures (CBIG-01-001)​

Ìý

±Ê°ù´Ç³¦±ð»å³Ü°ù²¹±ô:​

• CBIG-02-001 PBMC Isolation from Whole Blood (Conventional Method) (CBIG-02-001)​
• CBIG-02-002 PBMC Isolation from Whole Blood (Leucosep Method) (CBIG-02-002)​
• CBIG-02-003 Serum Isolation from Whole Blood ()​
• CBIG-02-004 DNA Extraction from Whole Blood (Accelerated Method) (CBIG-02-004)​
• CBIG-02-005 DNA Extraction from Whole Blood (Conventional Method) (CBIG-02-005)​
• CBIG-02-006 DNA Extraction from Saliva (CBIG-02-006)​Ìý
• CBIG-02-007 DNA Quantification through NanoDrop Spectrophotometry (CBIG-02-007)​
• CBIG-02-008 CSF Processing (CBIG-02-008)​
• CBIG-02-009 Preparation and Testing of Human AB Serum for Subsequent Storage and Use (CBIG-02-009)​
• CBIG-02-010 Thawing of Cryopreserved Cells (CBIG-02-010)​
• CBIG-02-011 Plasma Isolation from Whole Blood (CBIG-02-11)
• CBIG-02-012 Skin Biopsy (Sampling Procedure, Transport and Culture) (CBIG-02-012)​
• CBIG-02-013 Fibroblast Culture (CBIG-02-013)​
• CBIG-02-014 DNA Extraction from cell pellet (in preparation)​
• CBIG-02-015 How to Use an Oragene-DNA Test Tube for the Collection of Saliva (CBIG-02-015)​

Ìý

·¡±ç³Ü¾±±è³¾±ð²Ô³Ù:​

• CBIG-03-001 Different Methods to count cells (CBIG-03-001)​
• CBIG-03-002 RPM Conversion (CBIG-03-002)​

Ìý

¹ó´Ç°ù³¾²õ:​

• CBIG-02-001 PBMC (Conventional Method)​
• CBIG-02-002 PBMC (Leucosep Method)​
• CBIG-02-003 Serum
• CBIG-02-004 DNA Blood (Accelerated Method)​
• CBIG-02-006 Saliva

Publications

´¡°ù³Ù¾±³¦±ô±ð²õ:​

Natural killerÌýcellsÌýregulateÌýTh17ÌýcellsÌýafterÌýautologousÌýhematopoieticÌýstemÌýcellÌýtransplantation forÌýrelapsingÌýremittingÌýmultipleÌýsclerosis. Darlington et al.ÌýFrontiersÌýinÌýImmunology, 2008​

​

±Ê´Ç²õ³Ù±ð°ù²õ:​

13thÌýConferenceÌýonÌýBiobanking, Vancouver 2019 – Open ScienceÌýClinical,ÌýBiological, Imaging andÌýGeneticÌýRepositoryÌý– An IntegratedÌýApproachÌýtoÌýBiobanking​

BioConnect,ÌýMontrealÌý2016 – Open ScienceÌýClinical,ÌýBiological, Imaging andÌýGeneticÌýRepositoryÌý– an IntegratedÌýApproachÌýtoÌýBiobanking​

​

OralÌýPresentations:​

AmericanÌýAcademyÌýofÌýNeurologyÌý(AAN), Toronto 2020 –ÌýClinical,ÌýBiological,ÌýImaging andÌýGeneticÌýRepositoryÌýC-BIGR; AnÌýintegratedÌýApproachÌýtoÌýBiobankingÌýin theÌýContextÌýof Open Science