Guideline for sample preparation:
- Electrospray (ES) compatible solvents: water, methanol, acetonitrile, propanol, ethanol, dichloromethane, chloroform, etc.
- Solvent additives compatible with ES: volatile salts/buffers/acids, eg. ammonium acetate, ammonium formate, ammonium bicarbonate, formic acid, acetic acid.
- Solvent additives/buffers NOT compatible with ES: Trifluoroacetic acid (TFA), Sodium dodecyl sulphate (SDS), Ethylenediaminetetraacetic acid (EDTA), involatile salts/buffers, eg sodium, chloride, phosphates, TRIS, HEPES, etc.