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McGill.CA / Integrated Quantitative Biology Initiative / EquipmentNavigation / Automation

Robotics SAGIAN System

Our SAGIAN automated system has recently been upgraded thanks to a large infrastructure grant from the Canadian Foundation of Innovation (CFI), awarded to the Integrated Quantitative Bioscience Initiative (IQBI). The robotic system now boasts a brand-new SCARA arm, the latest Biomek pinning-liquid handler, a new Thermocycler and a high-performance CCD camera in a custom-made black box.Ìý It can perform (almost) any experiment, including liquid handling, colony pinning, shaking, incubating, sealing, running PCR, imaging over long periods of time...

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Module Description

Automated Workstation (Biomek NEW FXp, Beckman Coulter)

- Versatile liquid handler for 96- or 384-well plates.
- Equipped with a 96-channel 200 μl head and a 8-independent channel head.
- Pintools for 96-, 384 and 768 formats
- Enclosed in an Iso class 5 cleanroom.
- Different modules can be installed such as a magnetic bead unit, a filtration unit, a magnetic stirrer, an orbital shaker, a heating unit and a circulating reservoir.
Incubator (Cytomat 6001, Kendro)

- Accommodates up to 125 standard plates and 30 deep-well plates
- Incubation from 10 to 50ºC.
- CO2 supply possible for cell culture
- Agar plates can be incubated (and manipulated) upside down to prevent condensation on lid.
Plate Reader (DTX880, Beckman Coulter)

- For absorbance, luminescence, fluorescence intensity, fluorescence polarization and time-resolved fluorescence.
- Heated chamber (from 4ºC above ambient up to 45ºC).
- Reads from top or bottom of 6- to 384-well plates.
- Current filter sets include 260, 340, 370, 405, 450, 485, 535, 595 and 620 nm for excitation and 535, 595 and 625 nm for emission.
- Can perform endpoint, multiple wavelength, kinetic, or area scans.

Grashopper3 USB 3.0 CCD Camera (Point Grey)

- 10-40mm high resolution varifocal lens
- Enclosed for dark environment.
- Two bulbs providing white light from top of the plate.
- Polarized filters to prevent light reflection
- Medium- to high-resolution pictures (JPEG, RAW format).
Plate Washer (ELx405 select, Biotek Instruments)

- Dispenses from top and washes from bottom.
- Flow rate is adjustable for sensitive assays.
Carousel (Beckman Coulter)

- Accommodates up to 90 standard plates and 40 deep-well plates or tip boxes.

Thermocycler (TRobot , Biometra)

- 96 or 384 well plate block

Robotic Arm (SCARA, Beckman Coulter)

- Slides on a ~2 meter rail to move plates or labware between devices.
Dispenser (Multidrop384, Titertek)

- 8-channel head to dispense 5 to 395 μl from a single or multiple reagent bottles
Lidding Station (Beckman Coulter)

- Accommodates up to 6 lids.
- Maintains the lid-plate association.
Plate Shaker (Beckman Coulter)

- Configured with 6-plate locations.
Plate Sealer (ALPS 300, from Abgene)

- Used to seal plates for storage or to prevent evaporation or contamination during incubation or shaking.
Ìý Tip Lift and Disposal Unit (Beckman Coulter)

- Accommodates up to 96 200 μl tip boxes.
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